干擾素γ（IFNg）活性蛋白應(yīng)用解析

干擾素γ(IFNg)活性蛋白

Active Interferon Gamma (IFNg)

IFN-G; IFG; IFI; INFr; IFN, Immune Interferon

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• 細(xì)胞因子

• 感染免疫

• 編號(hào)APA049Hu01

• 物種Homo sapiens (Human，人)

• 品牌商.(美國(guó))

• 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）

• 性狀凍干粉

• 純度> 95%

• 等電點(diǎn)9.6

• 應(yīng)用Cell culture; Activity Assays; In vivo assays.

• 下載英文說明書   中文說明書

• 規(guī)格10ug50ug250ug1mg1g

 

活性實(shí)驗(yàn)

 



IFN-γ is a dimerized soluble cytokine that is the only member of the type II class of interferons. The importance of IFNγ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. As reported, IFNγ is an important activator of human monocytic THP1 cells. Therefore, THP-1 cells were incubated in RPMI 1640 with various concentration of IFN-γ, then cells were observed by inverted microscope everyday. After stimulated with IFN-γ (2ng/mL) for 5 days, morphological changes occurred in THP1 cells which displayed the shape of fusiform or polygon and were more likely to adhere. Effect of IFN-γ on THP1 cells is shown in Figure 1.

 

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

γ干擾素(IFN-γ)ELISA試劑盒【原理介紹】IFNγ，γ干擾素。和α,β干擾素并無明顯同源性。由受同種抗原、腫瘤、和分裂因子刺激的活T細(xì)胞NK細(xì)胞所分泌。IFNγ具有一系列的生物學(xué)功能：抗病毒作用，抑制腫瘤細(xì)胞生長(zhǎng)，促進(jìn)B細(xì)胞產(chǎn)生抗體。此外，IFNγ活巨噬細(xì)胞，增強(qiáng)NK細(xì)胞的細(xì)胞毒作用，刺激T細(xì)胞的細(xì)胞毒作用。

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