【文獻標題】Novel insights into cytochrome P450 enzyme and solute carrier families in cadmium-induced liver injury of pigs 

【作者】Huan Wang, Qi Han,Yongjie Chen,et.al

【作者單位】東北農(nóng)業(yè)大學（Northeast Agricultural University）

【文獻中引用產(chǎn)品】

豬丙氨酸轉氨酶(ALT)ELISA試劑盒

豬天門冬氨酸氨基轉移酶（AST)ELISA試劑盒

【關鍵詞】Cadmium ,Cytochrome P450 enzyme family ,Solute carrier family ,Pig ,Transcriptomics 

【DOI】doi.org/10.1016/j.ecoenv.2021.111910 

【影響因子(IF)】6.2

【出版期刊】《Ecotoxicology and Environmental Safety 》

【產(chǎn)品原文引用】

Serum isolated from anterior vena cava blood was used to determine alanine transaminase (ALT) and aspartate transaminase (AST) activities by Microplate assay kits (Shanghai Hengyuan Bioengineering Co., Ltd., China) according to manufacturer’s protocol. Serum ALT and AST levels were determined by double antibody sandwich method. First, the purified ALT or AST antibody of pigs was coated with the microporous plate, and then serum was added to the micropore to form the antibodyantigen-elisa antibody complex. After thorough washing, substrate TMB was added for color development. The OD values were measured by an enzyme-labeled instrument at the wavelength of 450 nm. The concentration of ALT or AST in the sample was calculated by the standard curve. 

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