【文獻標題】Interactions between phosphorus availability and microbes in a wheat–maize double cropping system: A reduced fertilization scheme 【作者】YU Xiao-jing, CHEN Qi, SHI Wen-cong, et.al 【作者單位】山東農業(yè)大學（Shandong Agricultural University） 【文獻中引用產品】 土壤酸性磷酸酶（ACP）ELISA試劑盒 土壤堿性磷酸酶（ALP）ELISA試劑盒 【關鍵詞】 wheat–maize rotation, Olsen-P, alkaline phosphatase, phosphorus fertilization, calcareous soils 【DOI】doi.org/10.1016/S2095-3119(20)63599-7 【影響因子(IF)】4.38 【出版期刊】《Journal of Integrative Agriculture》 【產品原文引用】 At the 12-leaf collar stage (V12) of maize in 2018, the following analyses were performed: (i) analysis of acid phosphatase (ACP) and ALP activity in the collected samples of fresh root and rhizosphere soil by using an ELISA Kit (Shanghai HengYuan Biological Technology Co., Ltd., Shanghai, China); (ii) analysis of ADP-glucose pyrophosphorylase (AGpase) of photosynthetic enzymes through enzyme-linked immunosorbent assay in the largest functional leaf samples collected; and (iii) determination of the net photosynthesis (Pn) rate of the largest functional leaf by using the LI-6400XT Portable Photosynthesis System (LI-Cor, Lincoln, NE, USA). After the harvest of each crop, the grain and straw were separated for the analysis of dry weight and total P (TP) content.
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